Resuscitation promoting factor E (RpfE) proteins in Mycobacterium tuberculosis (M. tuberculosis) may be recognized by the host immune system owing to their secretory nature. In this study, the RpfE protein was purified using DEAE–Sepharose column chromatography from M. tuberculosis H37Rv extracellular proteins. An upregulated production of gamma interferon (IFN- γ), interleukin-10 (IL-10) and IL-12 by splenocytes suggest T cell proliferation stimulated by purified RpfE protein. The present findings imply that the RpfE protein produced extracellularly in M. tuberculosis H37Rv can be used as a viable subunit vaccine candidate.
Alsahag, M. (2021). Mycobacterium tuberculosis RpfE Protein Purified from H37Rv Culture Filtrate Proteins Stimulates Splenocytes. Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology, 13(2), 35-44. doi: 10.21608/eajbse.2021.209694
MLA
Mansoor Alsahag. "Mycobacterium tuberculosis RpfE Protein Purified from H37Rv Culture Filtrate Proteins Stimulates Splenocytes". Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology, 13, 2, 2021, 35-44. doi: 10.21608/eajbse.2021.209694
HARVARD
Alsahag, M. (2021). 'Mycobacterium tuberculosis RpfE Protein Purified from H37Rv Culture Filtrate Proteins Stimulates Splenocytes', Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology, 13(2), pp. 35-44. doi: 10.21608/eajbse.2021.209694
VANCOUVER
Alsahag, M. Mycobacterium tuberculosis RpfE Protein Purified from H37Rv Culture Filtrate Proteins Stimulates Splenocytes. Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology, 2021; 13(2): 35-44. doi: 10.21608/eajbse.2021.209694
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