Molecular Differentiation of Haemonchus longistipes Railliet & Henry, 1909 (Nematoda: Trichostrongyloidea) of Female Morphotypes and Male Worms Infecting the Dromedary Camelus dromedarius Using RAPD Technique

Elsayed, Ayman N.; Badawy, Ahmed I.I.; Abdel-Aziz, Ahmed; Sarhan, Moustafa

doi:10.21608/eajbse.2019.48766

Molecular Differentiation of Haemonchus longistipes Railliet & Henry, 1909 (Nematoda: Trichostrongyloidea) of Female Morphotypes and Male Worms Infecting the Dromedary Camelus dromedarius Using RAPD Technique

Document Type : Original Article

Authors

¹ Zoology Department, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt

² Parasitology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig City 44511, Sharkia, Egypt.

³ Zoology Department, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt.

⁴ Molecular Biology Lab., Zoology Department, Faculty of Science, Al Azhar University, Assiut City 71524, Assiut, Egypt.

10.21608/eajbse.2019.48766

Abstract

Molecular variability among female morphotypes (smooth, knobbed and linguiform B) and male of Haemonchus longistipes from Camelus dromedarius were analyzed by means of random amplified polymorphic DNA (RAPD) using 5 selected decamer primers. Results showed that primers OPA-04 and OPC-1 showed monomorphic bands for female morphotypes and male worms. In contrast, primers OPB-03, OPB-18, and OPD-05 yielded polymorphic bands among female morphotypes and male worms. A number of bands amplified by each primer varied from two to ten bands and ranged from 200 to 1750 bp. Band frequency per H. longistipes worm and per primer was estimated. The neighbor-joining clustering data based on pairwise distances among female morphotypes and male indicated that each sample had its own genetic peculiarity.

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