Effect of Some Plant Extracts on The Biochemical Parameters, AChE and GST Activities of The Mosquito, Culex pipiens L. (Diptera: Culicidae)

Shahat, Mohamed A.M.; El-Sheikh, Tarek M.Y.; Hammad, Kotb M.; Hasaballah, Ahmed I.; Shehata, Ahmed Z.I.

doi:10.21608/eajbse.2020.128498

Effect of Some Plant Extracts on The Biochemical Parameters, AChE and GST Activities of The Mosquito, Culex pipiens L. (Diptera: Culicidae)

Document Type : Original Article

Authors

Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11651, Egypt.

10.21608/eajbse.2020.128498

Abstract

The present study was undertaken in order to investigate the effect of median lethal concentration (LC₅₀) of methanol, ethyl acetate, chlorobenzene and hexane extracts from leaves of Origanum syriacum, Pergularia tomentosa, Senna italica, and Otostegia fruticose against different biological aspects of the 3^rd instar larvae of Culex pipiens. The effects were examined on the biochemical composition of the larval body and the activities of acetylcholinesterase (AChE) and glutathione S-transferase (GST). Based on obtained results, hexane extract showedmore activity against 3^rd instar larvae of C. pipiens followed by chlorobenzene, ethyl acetate, and methanol extract. Tested extracts caused a modification in biochemical composition. The LC₅₀ of each treatment decreased the total carbohydrate, protein, and lipid contents as compared with the control. Tested extracts also showed a neurotoxic activity as evidenced by the inhibition effect of the AChE activity that was significantly (P<0.001) decreased. Stimulation of the detoxification system of tested larvae was evidenced by the promoted activity of GST as compared with the untreated group.In general, tested extracts used in the present study may be considered as promising control agents against the mosquito vector, C. pipiens.

Keywords