Egyptian Society of Biological SciencesEgyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology2090-07832120101201Species identification and infectivity rate of malaria vector in two endemic malaria areas in Sudan1151645510.21608/eajbse.2010.16455ENTasneem Moawia Abd AllahOsmanDepartment of Zoology, Faculty of Science, University of Khartoum.Journal Article20181013Attempts were made to identify, Anopheline species by using Polymerase Chain Reaction (PCR) and to determine the sporozoite infection rate in malaria vectors using Enzyme Linked Immunosorbent Assay (ELISA). Samples were collected from highly endemic areas in central Sudan (Sennar), and eastern Sudan (Koka).Morphological Identification was carried using available keys. <em>Anopheles gambiae </em>complexwas then identified using PCR.Out of fifty two was identified as <em>Anopheles arabiensis</em> (92.3 %). The sporozoite infection rate was estimated using ELISA method the infectivity rates were 16% (4/24), 15% (3/21) in Sennar and Koka respectively. This data indicated that the two areas are endemic for malaria and <em>An. arabiensis</em> is the main vector in the study areas. The sporozoite infection rate was found to be 16% (4/24) in Sennar samples, whereas it was found to be 15% (3/21) in Koka samples. Thus, data indicate of these two areas are endemic for malaria and <em>An.arabiensis</em> is the main vector in these areas.https://eajbse.journals.ekb.eg/article_16455_b6648ede3699fe936c320259ec496f93.pdfEgyptian Society of Biological SciencesEgyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology2090-07832120101201Light and Scanning Electron Microscopic Studies of Trypanosomes Parasites Infecting Freshwater Fishes in Qena Governorate, Egypt17311645610.21608/eajbse.2010.16456ENHussein A. N.A.-Zoology Department, Faculty of Science, South Valley University. Qena, Egypt
-Biology Department, Faculty of Science, King Khalid University. Abha, Kingdom of Saudi ArabiaRabie S.A.- Zoology Department, Faculty of Science, South Valley University. Qena, Egypt
- Faculty of Applied Sciences, Umm Al-Qura University, Saudi ArabiaMohammed N.E.Zoology Department, Faculty of Science, South Valley University. Qena, EgyptHussein N.M.Zoology Department, Faculty of Science, South Valley University. Qena, EgyptJournal Article20181013Freshwater fishes were sampled from different localities in the River Nile at Qena Governorate (Qus, Qift, Dandara, El-Trammsa, El-Maana, Dishna and Nag-Hammady). Investigation revealed that <em>Trypanosoma</em> were found parasitized blood of many fish species. Out of 581 blood smears examined, 143 revealed trypanosomes infection with a prevalence of 24.6%. The infected fishes are belonging to seven species and four families. Trypanosomes parasites were found belonged to three species with different infection rates: <em>T. mukasai</em> Van As <em>et al</em>. 2004 (28.3%); <em>T. cyanophilum</em> Mohamed, 1978 (52.9%) and <em>T. cataractae</em> Putz, 1972 (1.5%). A new <em>Trypanosoma</em> species was found infecting <em>Ch. ruppelli</em> fish with an infection rate of 1.96%. Histological sections showed the presence of the adult stage of <em>T. mukasai</em> in between liver cells. Also <em>T. mukasai</em> had been examined using scanning electron microscope.https://eajbse.journals.ekb.eg/article_16456_98578e00493096681832b28f51c98786.pdfEgyptian Society of Biological SciencesEgyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology2090-07832120101201Protein patterns of Admiral- treated and untreated house dust mites: Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acarina: Pyroglyphidae)33391645710.21608/eajbse.2010.16457ENNadiaHelmyDepartment of Entomology, Faculty of Science, Ain Shams University.Yousrya M.Abdel-HamidResearch Institute of Medical Entomology, Ministry of HealthAshraf A.AwadDepartment of Entomology, Faculty of Science, Ain Shams UniversityMohamed A.KenawyDepartment of Entomology, Faculty of Science, Ain Shams UniversityAkila M.El-ShafeiDepartment of Entomology, Faculty of Science, Ain Shams UniversityYousry Z. A.El-ZoheryDepartment of Haematology and Immunology, Faculty of Medicine, Al-Azhar University, Cairo, A.R. EgyptJournal Article20181013The present work deals with the study of the effect of Admiral "pyriproxyfen” as a juvenile hormone analogue (JHA) on the protein patterns of the two common house dust mites (HDM): <em>Dermatophagoides pteronyssinus</em> and <em>D. farinae</em>. Analysis revealed lower protein content in either JHA-treated or untreated tritonymphs than in the respective adults. JHA treatment affected protein concentration of both tritonymphs (significantly increased) and adults (significantly decreased). Significantly lower protein contents were detected for <em>D. farinae</em> than for <em>D.pteronyssinus</em> either in normal or JHA-treated individuals. A total of 49 bands with molecular weights of 10-108 Kilo Daltons (KD) and relative mobilities of 0.99-0.16 were electrophoretically separated from the whole body tissue extracts of normal and treated tritonymphs and adults of the two species. Four common bands (12-60 KD) were suggested as genus-specific. Three <em>D. pteronyssinus</em> proteins (21-33 KD) and one <em>D. farinae</em> protein (69 KD) were suggested as species- specific. The HDM allergen (14-15 KD) was detected in the two mite species. Several proteins that detected in normal individuals of the two species disappeared in the respective treated individuals that may be due to the effect of treatment with such JHA.https://eajbse.journals.ekb.eg/article_16457_110d16f23f78eace1883afbd75143d90.pdf