Species identification and infectivity rate of malaria vector in two endemic malaria areas in Sudan
Tasneem
Osman
Department of Zoology, Faculty of Science, University of Khartoum.
author
text
article
2010
eng
Attempts were made to identify, Anopheline species by using Polymerase Chain Reaction (PCR) and to determine the sporozoite infection rate in malaria vectors using Enzyme Linked Immunosorbent Assay (ELISA). Samples were collected from highly endemic areas in central Sudan (Sennar), and eastern Sudan (Koka).Morphological Identification was carried using available keys. Anopheles gambiae complexwas then identified using PCR.Out of fifty two was identified as Anopheles arabiensis (92.3 %). The sporozoite infection rate was estimated using ELISA method the infectivity rates were 16% (4/24), 15% (3/21) in Sennar and Koka respectively. This data indicated that the two areas are endemic for malaria and An. arabiensis is the main vector in the study areas. The sporozoite infection rate was found to be 16% (4/24) in Sennar samples, whereas it was found to be 15% (3/21) in Koka samples. Thus, data indicate of these two areas are endemic for malaria and An.arabiensis is the main vector in these areas.
Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology
Egyptian Society of Biological Sciences
2090-0783
2
v.
1
no.
2010
1
15
https://eajbse.journals.ekb.eg/article_16455_b6648ede3699fe936c320259ec496f93.pdf
dx.doi.org/10.21608/eajbse.2010.16455
Light and Scanning Electron Microscopic Studies of Trypanosomes Parasites Infecting Freshwater Fishes in Qena Governorate, Egypt
Hussein
A.
-Zoology Department, Faculty of Science, South Valley University. Qena, Egypt
-Biology Department, Faculty of Science, King Khalid University. Abha, Kingdom of Saudi Arabia
author
Rabie
A.
- Zoology Department, Faculty of Science, South Valley University. Qena, Egypt
- Faculty of Applied Sciences, Umm Al-Qura University, Saudi Arabia
author
Mohammed
E.
Zoology Department, Faculty of Science, South Valley University. Qena, Egypt
author
Hussein
M.
Zoology Department, Faculty of Science, South Valley University. Qena, Egypt
author
text
article
2010
eng
Freshwater fishes were sampled from different localities in the River Nile at Qena Governorate (Qus, Qift, Dandara, El-Trammsa, El-Maana, Dishna and Nag-Hammady). Investigation revealed that Trypanosoma were found parasitized blood of many fish species. Out of 581 blood smears examined, 143 revealed trypanosomes infection with a prevalence of 24.6%. The infected fishes are belonging to seven species and four families. Trypanosomes parasites were found belonged to three species with different infection rates: T. mukasai Van As et al. 2004 (28.3%); T. cyanophilum Mohamed, 1978 (52.9%) and T. cataractae Putz, 1972 (1.5%). A new Trypanosoma species was found infecting Ch. ruppelli fish with an infection rate of 1.96%. Histological sections showed the presence of the adult stage of T. mukasai in between liver cells. Also T. mukasai had been examined using scanning electron microscope.
Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology
Egyptian Society of Biological Sciences
2090-0783
2
v.
1
no.
2010
17
31
https://eajbse.journals.ekb.eg/article_16456_98578e00493096681832b28f51c98786.pdf
dx.doi.org/10.21608/eajbse.2010.16456
Protein patterns of Admiral- treated and untreated house dust mites: Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acarina: Pyroglyphidae)
Nadia
Helmy
Department of Entomology, Faculty of Science, Ain Shams University.
author
Yousrya
Abdel-Hamid
Research Institute of Medical Entomology, Ministry of Health
author
Ashraf
Awad
Department of Entomology, Faculty of Science, Ain Shams University
author
Mohamed
Kenawy
Department of Entomology, Faculty of Science, Ain Shams University
author
Akila
El-Shafei
Department of Entomology, Faculty of Science, Ain Shams University
author
Yousry
El-Zohery
Department of Haematology and Immunology, Faculty of Medicine, Al-Azhar University, Cairo, A.R. Egypt
author
text
article
2010
eng
The present work deals with the study of the effect of Admiral "pyriproxyfen” as a juvenile hormone analogue (JHA) on the protein patterns of the two common house dust mites (HDM): Dermatophagoides pteronyssinus and D. farinae. Analysis revealed lower protein content in either JHA-treated or untreated tritonymphs than in the respective adults. JHA treatment affected protein concentration of both tritonymphs (significantly increased) and adults (significantly decreased). Significantly lower protein contents were detected for D. farinae than for D.pteronyssinus either in normal or JHA-treated individuals. A total of 49 bands with molecular weights of 10-108 Kilo Daltons (KD) and relative mobilities of 0.99-0.16 were electrophoretically separated from the whole body tissue extracts of normal and treated tritonymphs and adults of the two species. Four common bands (12-60 KD) were suggested as genus-specific. Three D. pteronyssinus proteins (21-33 KD) and one D. farinae protein (69 KD) were suggested as species- specific. The HDM allergen (14-15 KD) was detected in the two mite species. Several proteins that detected in normal individuals of the two species disappeared in the respective treated individuals that may be due to the effect of treatment with such JHA.
Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology
Egyptian Society of Biological Sciences
2090-0783
2
v.
1
no.
2010
33
39
https://eajbse.journals.ekb.eg/article_16457_110d16f23f78eace1883afbd75143d90.pdf
dx.doi.org/10.21608/eajbse.2010.16457